{"product_id":"easy-af-agar-pots","title":"Basics: Easy AF Agar Pots 4pk","description":"\u003cp\u003eThis might be one of the coolest products we've added in a while.\u003c\/p\u003e\n\u003cp\u003eWe asked our Discord community what we should call these so out of respect for our unhinged customer base I'll include the names they suggested at the bottom.\u003c\/p\u003e\n\u003cp\u003eThe product itself isn't anything particularly new. It's basically a modified pp5 pot with high nutrient agar. It has a Microppose injection port and filter to allow gas exchange.\u003c\/p\u003e\n\u003ch3\u003eWhy is it cool?\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eGerminating spores\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFirstly it gets you using agar if you've never tried it, you can inject a few drops of spore solution through the port and watch it grow out on the agar, the high nutrient mix shows contamination.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLiquid Culture\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis is where it gets really cool. The typical process for liquid creation is to create sterile liquid culture media and transfer your mycelium from agar to the LC once the plate has colonised nicely. This requires some aseptic technique. At a bare minimum a still air box.\u003c\/p\u003e\n\u003cp\u003eI thought recently, why not do it the other way around?\u003c\/p\u003e\n\u003cp\u003eSo we added 20ml of sterile LC media to the pot after the mycelium had established. Sure enough, 2 days later we had mycelium growing in the liquid on the surface of the agar. We shook it up, then drew up the liquid in a syringe and transferred it back into sterile jar of media. \u003c\/p\u003e\n\u003cp\u003eWithout any aseptic technique we'd made a liquid culture from spores using the correct(ish) method using agar.\u003c\/p\u003e\n\u003cp\u003eI'm sure someone will tell me ShitBum_2000 on the Shroomery did this first in '99, but until then I'm claiming this as \u003cstrong\u003eMYCOPUNKS LC KETCHUP POT TEK \u003csup\u003etm\u003c\/sup\u003e\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp\u003eIt doesn't stop there...\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNeedle Biopsy Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eWe've successfully cloned mushrooms using a large gauge needle to biopsy mycelium from a mushroom and put it directly through the injection port onto agar.\u003c\/p\u003e\n\u003cp\u003eThis could be especially cool for collecting mycelium in the field for cleaning up later.\u003c\/p\u003e\n\u003cp\u003eSo now we've cloned, germinated and created liquid culture with no still air box.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCustomer name suggestions (mental) - sorry in advance:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMushwombs (actually quite clever)\u003c\/li\u003e\n\u003cli\u003eCumpots\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003ePotsMyco \u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eCum Chalice\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003edonor dumps\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003ePunkplates\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003es(PunkPots)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003ePots of Glory - With Holes\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eFractal Fuel\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSplooge Pots\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003ePunk Holes - \"ready for a quick prick\"\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eEZ-Clean plates\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSporegasms\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eGrowgurt\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003ePot luck\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eMycobrew\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003ePetit Agars\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSperm Sample Pot\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSpunknoculation Pots\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eCum Buckets\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eLil Chubs\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eCM pots\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eUncle Chris’s gelatinous prick vessel.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSporeplay\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eShank-n-wank tanks\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eKumpot\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eMycoMuffs\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003estab and squirt pot\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"MycoPunks","offers":[{"title":"Default Title","offer_id":56190586814845,"sku":"AgarPots4pk","price":8.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0542\/6721\/6063\/files\/EasyAF_Agar_Pots.webp?v=1751016140","url":"https:\/\/mycopunks.com\/fi\/products\/easy-af-agar-pots","provider":"MycoPunks","version":"1.0","type":"link"}